The Transcriptional Landscape of Hematopoietic Stem Cell Ontogeny

Hematopoietic stem cells (HSCs) are are born in the aorta-gonads-mesonephros (AGM) around 10.5 post conception and migrate to the fetal liver (FL), placenta, and eventually the bone marrow, where they reside for the life of the adult organism. To identify regulators of the specification and development of HSCs, we performed gene expression profiling of highly purified HSCs and HSC precursors at all major stages of development, as well as HSCs derived from embryonic stem cells and their in vitro progenitors. Illustrated below are the sources of the cell populations profiled. We applied a Systems Biology approach to first find modules of co-regulated genes and second, to find the transcriptional regulators of the modules.

Differential Expression	Modules of co-expressed genes	Transcriptional regulators of HSC development

This website provides a portal to explore and mine this unique dataset and analysis of the developing HSC. Visitors of the site can use it to:

• Examine the expression pattern of gene(s) of interest during HSC ontogeny
• Find putative transcriptional regulators of the transcriptional programs underlying HSC development
• Find genes that are differentially expressed during HSC ontogeny
• Download processed data and analysis results